The Robins recorded their first league win of 2024 last time out as goals from Jason Knight and Matty James saw them edge past Middlesbrough.
Liam Manning will be hoping that result will turn City's fortunes around, after a slow start to the new year in terms of points, with no wins in five before their win over Michael Carrick's side.
They took West Ham to a replay before knocking the Hammers out in the return tie at Ashton Gate, before being narrowly defeated by Nottingham Forest on penalties after a well-fought 210 minutes of football against the topflight side across two matches.
Manning will be boosted by the potential return of several key players to the side, including Scott Twine who netted in his only game for the club so far, and Mark Sykes.
Rob Atkinson is also making progress and featured for the u21s on Saturday, as is Kal Naismith, but the match will come too soon for the pair.
While City will want a positive result, they will face no easy task.
Russell Martin's side sit in second place in the league table and are on a remarkable unbeaten run of 25 matches in all competitions.
The last time these two met, Southampton ran out narrow 1-0 winners, and City will want revenge on Tuesday.
Here's how I think the Robins will set up...